They were created by Gumball and Darwin. They are clones of the original Anton, except for the fact that they are extremely stupid. A notable clone is Ant-Three, who was created by Darwin when Ant-Two disappeared.
The Anton clones are not very smart, and are easily manipulated. They seem to follow whoever is superior to them (like Gumball and Darwin initially when they were created, then even the notorious Ant-One after he knocks out Richard). They are also determined in obeying their master, even if it means their death (such as when they jumped into a lake, or fought Darwin despite their disadvantage in strength and size).